Mo-MLO-04 - Centrifugal Microfluidic Automation of Protein Sample Preparation for Nanopore Sensing

Abstract

We present a microfluidic LabDisk automating the solid-phase-enhanced sample preparation (SP3) workflow for nanopore sensing.Using myelin basic protein (MBP), the system produces nanopore-compatible samples preserving membrane stability and enabling reliable detection with the aerolysin AeL-R220S nanopore variant.A distinct MBP peptide marker supports confident identification.Automated and manual workflows show identical nanopore signals (I/I₀ ≈ 0.75, <0.2 % deviation) and comparable missed cleavage rates, demonstrating robust, standardized, and user-friendly sample preparation.