What comes after de novo ? Automated lead optimization of proteins with CRADLE-1

Abstract

ABSTRACT Lead optimization remains the longest and most expensive step in pre-clinical drug discovery, typically consuming 12-36 months whilst costing $5M-$15M per candidate. We introduce ‘CRADLE-1’, an automated framework for protein engineering. While CRADLE-1 supports the full process of drug discovery and industrial protein engineering pipelines, including hit identification and de nouo binder design, this work focuses on its application to multi-property lead optimization across protein modalities (VHHs, scFvs, IgGs, peptides, enzymes, CRISPR systems, vaccines). We show it is 4-7× faster than rational design, as measured by the number of wet lab rounds required. We provide in-vitro validation across all of the above modalities, typically optimizing multiple properties simultaneously (single and polyspecific binding down to picomolar, activity, thermostability,.). Technically, CRADLE-1 starts with pre-trained foundation protein language models (PLMs), which are fine-tuned in unsupervised fashion on evolutionary neighborhoods, in supervised fashion using lab-in-the-loop data, and then deployed in a multi-model workflow. Of additional interest, we find that (a) the end-to-end system may be run in automated fashion; (b) wet lab data may be consumed in ‘black box’ fashion without knowledge of the underlying biochemical mechanisms; (c) structural data may largely be superseded by sequence-function pairs.