Self-assembling protein cages: from coiled-coil module to machine learning-driven de novo design of next-generation biomaterials

Abstract

protein cages has recently been revolutionized by new concepts of modular protein design, computational design of interacting surfaces and machine learning-based generative protein design. Protein cages can be designed in diverse architectures and sizes, and their assembly and disassembly can be regulated by chemical, biological, and physical signals. Here, we focus on the review of engineering strategies for the designed protein cages based on coiled coils or other modular protein domains, their functionalization and opportunities of customized engineered protein cages.